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Updated: Aug 6, 2026

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Standardized Trait Measurement in Maize Breeding and Genetics Research
Dayane Cristina Lima1, Natalia de Leon2, Shawn M Kaeppler3,2
1Wisconsin Crop Innovation Center, University of Wisconsin-Madison, Middleton, Wisconsin 53562, USA dclima@wisc.edu.
Abstract:
Maize (Zea mays L.) is a highly versatile crop, grown worldwide under different climatic conditions and management practices. Its wide-ranging uses across multiple sectors, including food and feed production, biofuels, and as a source of raw materials for industrial processes, make the traits of interest highly variable, depending on research and breeding goals and the needs of commercial and public end users. The phenotype observed in these contexts reflects the combined effects of the genotype, the environment, and their interaction, making multienvironment trials crucial for identifying superior cultivars and predicting their performance across diverse conditions. Accurate and consistent trait measurements and recording are therefore essential to enable meaningful comparisons and integration of data across years, locations, and studies. This is particularly important in the public sector, where such multienvironment trails are often only feasible primarily through collaborative efforts. Here, we provide a brief overview of the importance of standardized trait measurements in maize research and breeding programs, and discuss their impact on enhancing the value and usability of publicly accessible phenotyping data. We also highlight how standardization helps overcome the challenges of conducting large-scale experiments across locations and years, particularly in resource-limited public programs, where multiinstitutional coordination is essential. Together, these approaches help advance reproducibility and data sharing across maize research programs.
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