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Updated: Aug 6, 2026
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Dot Blot Assay for Detecting Global N6-Methyladenosine RNA Modification Levels
Published on: February 6, 2026
SERPINE1 Alternative Polyadenylation Influences Gastric Cancer Prognosis via N6-Methyladenosine Modification
Zhuoyi Wu1, Ruyun Xu1, Ju Fang2
1Department of Epidemiology and Biostatistics, Center for Big Data and Population Health of IHM, School of Public Health, Anhui Medical University, Hefei, Anhui, China.
Cancer Science
|July 21, 2026
Summary
Alternative polyadenylation and m6A methylation synergistically regulate SERPINE1 in gastric cancer. This interaction promotes tumor malignancy, identifying SERPINE1 as a prognostic biomarker.
Area of Science:
- Molecular Biology
- Cancer Research
- Genomics
Background:
- Alternative polyadenylation (APA) and N6-methyladenosine (m6A) methylation are key post-transcriptional regulators in cancer.
- Their combined role in gastric cancer (GC) pathogenesis is not well understood.
Purpose of the Study:
- To investigate the synergistic role of APA and m6A methylation in gastric cancer.
- To identify novel prognostic biomarkers and therapeutic targets in GC.
Main Methods:
- Integrated analysis of RNA-seq and MeRIP-seq data from GC tissues.
- Identification of APA events and m6A methylation sites.
- Co-immunoprecipitation, gene silencing, and functional assays.
Main Results:
- Identified 9769 APA events and 3117 differentially methylated sites, with 165 genes showing concurrent alterations.
- SERPINE1 was identified as an independent prognostic factor in GC, with high expression linked to advanced stages and poor survival.
- CSTF2 and IGF2BP1 were found to cooperatively regulate SERPINE1 3'UTR shortening, enhancing GC cell proliferation, migration, and invasion.
Conclusions:
- CSTF2 and IGF2BP1 synergistically promote SERPINE1 3'UTR shortening, increasing GC malignancy.
- SERPINE1 is a potential prognostic biomarker and therapeutic target for gastric cancer.
