Related Experiment Video
Updated: Aug 6, 2026
10:22
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
Published on: October 31, 2025
Spatial Total RNA Sequencing of Formalin-Fixed Paraffin-Embedded Tissue by spRandom-seq
Yuan Liao1,2, Jiaye Chen1, Shunji Zhang3
1Department of Laboratory Medicine of The First Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, China.
Small (Weinheim an Der Bergstrasse, Germany)
|July 21, 2026
Summary
spRandom-seq enables spatial total RNA sequencing in FFPE tissues, resolving host, bacterial, and viral transcripts. This technology reveals spatial heterogeneity in cancers and infections, aiding clinical pathology and diagnostics.
Area of Science:
- Molecular Biology
- Genomics
- Pathology
Background:
- Understanding the molecular pathogenesis of diseases requires analyzing RNA transcripts within the tissue microenvironment.
- Spatially resolving comprehensive transcriptional landscapes in complex clinical tissues like FFPE specimens remains challenging.
Purpose of the Study:
- To develop a spatial total RNA sequencing technology (spRandom-seq) for FFPE tissues.
- To enable the spatial resolution of host, bacterial, and viral RNA transcripts.
Main Methods:
- spRandom-seq utilizes a random primer-based approach for spatial total RNA sequencing.
- The technology is compatible with existing spatial transcriptomics platforms.
Main Results:
- spRandom-seq identified lncRNAs and alternative splicing events in mouse brain.
- It revealed spatial heterogeneity in breast cancer FFPE sections and microbial infection sites.
- Integrated analysis in HBV-positive HCC showed activated complement/coagulation pathways and increased CNVs in HBV-infected areas.
Conclusions:
- spRandom-seq is a practical and scalable approach for analyzing FFPE tissues.
- It offers potential for clinical pathology applications and infection diagnostics.
