Integrated Kinetic and Transcriptomic Insights Into Nano-Assisted Tandemol Biodegradation by UV-Mutagenized...

Area of Science:

• Environmental Microbiology
• Bioremediation
• Biotechnology

Background:

• Petroleum-based lubricants like Tandemol pose environmental challenges.
• Bioremediation using microorganisms offers a sustainable solution for lubricant degradation.
• Optimizing microbial degradation requires understanding influencing factors like UV treatment and nanoparticle inclusion.

Purpose of the Study:

• To investigate the kinetics of Tandemol lubricant degradation by Pseudomonas synaxantha rhizo-25.
• To evaluate the impact of UV treatment and nanoparticle inclusion on degradation efficiency.
• To identify key enzymes and metabolic pathways involved in the degradation process.

Main Methods:

• Assessed degradation of Total Petroleum Hydrocarbons (TPH) and aromatic compounds.
• Utilized Gas Chromatography-Mass Spectrometry (GC-MS) for metabolite profiling.
• Employed transcriptomic analysis to identify key enzymes and degradation pathways (meta-cleavage).

Main Results:

• UV treatment and nanoparticle inclusion accelerated TPH and aromatic compound degradation rates.
• Biodegradation half-lives were significantly reduced for both TPH and aromatic compounds under optimized conditions.
• Identified Cytochrome P450 and Tau D/Tfd A dioxygenase as key enzymes in the aromatic degradation pathway.

Conclusions:

• UV treatment and nanoparticle supplementation markedly enhance the bioremediation efficiency of Tandemol lubricant.
• Pseudomonas synaxantha rhizo-25 demonstrates significant potential for petroleum contaminant cleanup.
• The meta-cleavage pathway, initiated by terminal oxidation, is crucial for aromatic compound breakdown.