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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
Novel red emission fluorescent carbon dots with narrow-bandwidth emission for nitrite detection in foods and cell
Huijing Hou1,2, Qian Du1, Yaqin Zhao1
1College of Chemistry and Chemical Engineering, Shanxi University, No. 36 Wucheng Road, Taiyuan, 030006, Shanxi Province, P. R. China.
Abstract:
Nitrite (NO2-) is widely present in water and commonly used in food products. Excessive ingestion of NO2- poses significant hazards to human health. Therefore, the development of accurate and sensitive nitrite detection methods is greatly essential for public health safety. Herein, novel red-emitting carbon dots (r-CDs) with a narrow emission width (45nm) were prepared through a simple hydrothermal reaction. The fluorescence of r-CDs at 628 nm could be directly and gradually quenched by NO2-. Thus, a highly sensitive and selective nitrite sensing platform was constructed with a wide linear concentration range of 3-200 µM and a detection limit of 0.85 µM. Satisfactory detection performance of NO2- was verified in meat, water, milk, and pickled vegetable samples. Furthermore, this probe was successfully applied in distinct fluorescence imaging, exhibiting great potential for the intracellular analysis of NO2-. In conclusion, this narrow-emission NO2- sensor features facile synthesis, good anti-interference capability, and a wide application scope.
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