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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
Published on: September 20, 2016
Mitochondrial engineering strategies in yeast cell factories
Shuo Yang1, Cong Gao1, Guipeng Hu2
1School of Biotechnology and Key Laboratory of Industrial Biotechnology of the Ministry of Education, Jiangnan University, Wuxi, China; Key Laboratory of Industrial Synthetic Biology of Jiangsu Province, Jiangnan University, Wuxi, China.
None:
Yeast is widely used as a microbial chassis for sustainable chemical production, with subcellular organelles helping to organize and regulate biosynthesis. Among these organelles, mitochondria play pivotal roles in yeast cell factories by supplying metabolic resources, maintaining cellular vitality, and providing a favorable biosynthetic microenvironment. Accordingly, this review summarizes mitochondria-centered strategies for improving yeast-based chemical production. These strategies include (i) rewiring mitochondrial metabolic pathways to regulate metabolic resource supply; (ii) maintaining mitochondrial homeostasis to improve cellular vitality; and (iii) optimizing mitochondrial compartmentalization. Current bottlenecks and future opportunities are discussed, providing a framework for optimizing chemical biosynthesis in yeast cell factories.
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