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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

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Hybridization chain reaction-assisted CRISPR/Cas12a strategy for rapid and visual detection of Haemophilus influenzae

Chunfang Ma1, Jiayi Zhang2, Yayun Jiang3

  • 1Department of Clinical Laboratory, Suzhou Ninth People's Hospital, Suzhou Ninth Hospital Affiliated to Soochow University, Suzhou, China.

Frontiers in Cellular and Infection Microbiology
|July 22, 2026
PubMed

Insights

A new visual CRISPR/Cas12a platform, Vi-CasHCP, rapidly detects Haemophilus influenzae. This sensitive and specific test offers a convenient alternative for diagnosing childhood pneumonia, especially in resource-limited settings.

Area of Science:

  • Molecular Biology
  • Diagnostics
  • Microbiology

Background:

  • Haemophilus influenzae causes severe childhood pneumonia.
  • Current detection methods (culture, qPCR) are slow and require complex equipment.
  • Rapid, sensitive, and specific diagnostics are crucial for effective treatment.

Purpose of the Study:

  • To develop a visual CRISPR/Cas12a-based detection platform for Haemophilus influenzae.
  • To improve diagnostic speed, sensitivity, and convenience for clinical applications.

Main Methods:

  • Integrated CRISPR/Cas12a with hybridization chain reaction (HCR) and HRP-catalyzed TMB colorimetric assay (Vi-CasHCP).
  • Utilized Recombinase polymerase amplification for enhanced sensitivity.
  • Validated performance using respiratory samples and compared with qPCR.

Main Results:

  • Vi-CasHCP achieved a detection limit of 11.8 CFU/mL in 70 minutes.
  • Demonstrated 100% sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) in clinical validation.
  • Provided a clear visual color change for easy interpretation.

Conclusions:

  • Vi-CasHCP offers rapid, sensitive, and specific detection of Haemophilus influenzae.
  • The platform requires no complex instrumentation, making it suitable for resource-limited settings.
  • Vi-CasHCP provides a valuable tool for fast and accurate diagnosis of childhood pneumonia.

Keywords:
CRISPR/Cas12aHaemophilus influenzaehybridization chain reactionrapidvisual detection

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