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Enhanced Extraction of Low-Molecular Weight DNA from Wastewater for Comprehensive Assessment of Antimicrobial Resistance
Published on: July 19, 2024
Environmental effectiveness of the National Action Plan to Contain Antimicrobial Resistance: evidence from Chinese
Yuxiang Zhao1,2,3, Zishu Liu2,3,4, Xi Chen1
1Environmental Microbiome Engineering and Biotechnology Laboratory, Center for Environmental Engineering Research, Department of Civil Engineering, The University of Hong Kong, Hong Kong 999077, China.
Abstract:
Soil antibiotic resistance genes (ARGs) represent an emerging planetary health threat. However, the environmental impacts of antimicrobial resistance (AMR) control policies remain unclear. Based on 2243 Chinese metagenomes, we generated a 14-year (2009-2022) spatiotemporal profile of Chinese soil ARGs and developed an open-access platform based on the interactive map. Relative to pre-2015 samples, the relative abundance of total ARGs (52.6%) and Rank I ARGs (77.0%) in croplands decreased markedly after 2016, coinciding with the national AMR control policy period (2016-2020). Comparing soil resistomes globally (2556 metagenomes) revealed homogenization in croplands, reflecting convergent ARG profiles under similar agricultural pressures across regions. This underscores the need for a shift from national to global intervention. Our findings highlight the importance of coordinated strategies that combined chemical pollution control with agricultural best practices to curb ARGs' dissemination under the One Health framework.
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