Induced diabetic neurogenic bladder animal models: application characteristics and standardization proposals via...

Area of Science:

• Urology
• Diabetology
• Neuroscience
• Animal Modeling

Background:

• Diabetic neurogenic bladder (DNB) is a significant complication of diabetes mellitus, potentially leading to severe renal damage.
• Current DNB animal models lack unified standards, hindering research progress and reliable data interpretation.
• Previous reviews have not provided sufficient objective data to guide the development of standardized DNB models.

Purpose of the Study:

• To systematically summarize the characteristics of existing Diabetic Neurogenic Bladder (DNB) animal models.
• To provide a theoretical reference for establishing standardized DNB animal models through data mining.
• To address the research gap concerning objective data in DNB animal model reviews.

Main Methods:

• Systematic literature search across 7 databases (PubMed, CNKI, etc.) following PRISMA guidelines.
• Inclusion of 352 eligible studies for analysis, with quality assessment using the SYRCLE tool.
• Data mining and statistical analysis using SPSS Modeler (Apriori algorithm) and R software for visualization.

Main Results:

• SD rats, Wistar rats, and C57BL/6 mice are predominant species; 82.39% of models used male animals.
• Chemical induction, primarily streptozotocin (STZ) (74.15%), is the main method, often combined with high-sugar/high-fat diets.
• Successful modeling criteria include blood glucose, urodynamics, and HbA1c; common STZ dose is 60 mg/kg over 8 weeks, but biases like insufficient blinding exist.

Conclusions:

• This study provides clarity on the current status of DNB animal models, filling a gap in existing literature.
• Future research should prioritize optimizing modeling protocols and promoting non-invasive detection methods.
• Enhancing standardized reporting of study details is crucial for improving the reliability and comparability of DNB animal models.