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Updated: Aug 6, 2026
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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
Published on: July 14, 2015
Hierarchically Structured Functionalized Polymer Monoliths Via Two-Photon Polymerization for Miniaturized HPLC
Tobias Drieschner1, Tobias Werres2, Martin Klassen2
1Lehr- und Forschungszentrum Process Analysis and Technology (LFZ PA&T), Reutlingen University, Alteburgstrasse 150, 72762Reutlingen, Germany.
Analytical Chemistry
|July 22, 2026
Summary
Additive manufacturing enables 3D printing of unique polymer monoliths for miniaturized liquid chromatography (LC). These hierarchically structured materials advance reversed-phase separation technologies.
Area of Science:
- Polymer Chemistry
- Chromatography
- Additive Manufacturing
Background:
- Miniaturized liquid chromatography (LC) systems offer advantages but are underutilized.
- Conventional polymer monolith synthesis limits control over pore structure.
- Hierarchical porosity is desirable for enhanced chromatographic performance.
Purpose of the Study:
- To develop hierarchically structured polymer monoliths using additive manufacturing.
- To apply these monoliths as stationary phases in miniaturized reversed-phase chromatography.
- To enable independent tuning of macropore and mesopore characteristics.
Main Methods:
- Utilized two-photon polymerization 3D printing to create Schoen gyroid macroporous structures.
- Developed a photoresin formulation with porogens for mesopore induction and hydrophobic monomers for reversed-phase functionality.
- Characterized monoliths using scanning electron microscopy and nano-computed tomography.
- Integrated the monolith into a microfluidic system for chromatographic separations.
Main Results:
- Successfully fabricated hierarchically structured polymer monoliths with tunable macropore geometry and mesoporosity.
- Demonstrated the material's suitability for reversed-phase chromatography.
- Achieved chromatographic separation of nonpolar analytes like naphthalene and fluoranthene.
Conclusions:
- Additive manufacturing provides precise control over hierarchical monolith structures for chromatography.
- Functionalized polymer monoliths are effective stationary phases for miniaturized reversed-phase LC.
- This approach overcomes limitations of conventional monolith synthesis, advancing microscale separation techniques.
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