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Updated: Aug 6, 2026
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Published on: October 20, 2018
A 3D RNA-protein proximity ligation protocol detecting the heterogeneity of LISRR-RPL26 interactions in
Alessandro Marino1, Eleonora Leucci2
1Laboratory for RNA Cancer Biology, Department of Oncology, LKI, KU Leuven, 3000 Leuven, Belgium.
Abstract:
Investigating lncRNA-protein interactors is crucial for understanding their contribution to tumor formation and progression. Here, we present a protocol to explore the heterogeneity of RNA-protein interactions between the lncRNA LISRR and RPL26 ex vivo in 3D clinically relevant patient-derived melanoma models. We describe steps for sample preparation, RNA probe hybridization, and a proximity ligation assay. We then detail procedures for detection and analysis. This protocol bypasses tissue embedding and sectioning, significantly accelerating data acquisition.
