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Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
Published on: May 3, 2014
Cleavage and Polyadenylation Specificity Factor 6-Mediated Alternative Polyadenylation of PSMC6 Is Critical for
Xuyong Qin1, Xiwen Guan1, Na Li1
1Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Xianyang712100, China.
Abstract:
Alternative polyadenylation within the 3' untranslated region (3' UTR-APA) is a post-transcriptional mechanism that influences mRNA stability and translation. Here, we investigated the role of 3' UTR-APA in regulating proteasome 26S subunit, ATPase 6 (PSMC6) in bovine Sertoli cells (BSCs). BSCs generated two PSMC6 mRNA isoforms through 3' UTR-APA, and the long 3' UTR isoform exhibited higher mRNA and protein levels than the short isoform. Further analyses revealed that cleavage and polyadenylation specificity factor 6 (CPSF6)-mediated APA regulation results in lower expression of the short isoform. Mechanistically, CPSF6 targets the UGUA motif upstream of the proximal polyadenylation signal (PAS) within the PSMC6 3' UTR, thereby biasing APA toward proximal PAS usage. This shift alters isoform ratios and mRNA stability, regulating BSC function. Collectively, our findings highlight the critical role of CPSF6-mediated PSMC6 3' UTR-APA in BSC function and provide a basis for marker-assisted breeding of male reproductive traits in cattle.
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