Chestnut shell polyphenol-loaded electrospun nanofiber-hydrogel dressing on chronic wounds in rats with type 2...

Multifunctional dressings have emerged as a promising approach for treating chronic wounds. a bionic, multifunctional hydrogel nanofiber dual-layered dressing (H-NF-DLD) were developed. The dressing was electrostatically spun with chestnut shell polyphenols (CSPs) and levofloxacin (LEV) loaded onto a zein nanofiber membrane (NFM), which was laminated with a sodium alginate and carboxymethyl chitosan crosslinked hydrogel layer to form a "sandwich" structure. The combination of CSPs and LEV resulted in significant responsiveness of the NFM in terms of stability, antioxidant properties, natural degradation, limited cytotoxicity, and hemolysis. The antimicrobial activity of the NFM against Staphylococcus aureus and Pseudomonas aeruginosa was >60%, higher than that of the LEV-only dressing (53%-56%). The wound healing time using the mixed CSP and LEV dressing (CSP-LEV) was advanced by 7 days compared with that in diabetic rats. The dressing was permeable and superior to commercial LEV alone. The healing effects of the dressing were related to its antioxidant, anti-inflammatory, bacteriostatic, and growth-promoting properties. This study provides an efficient therapeutic strategy for wounds in type 2 diabetes. The developed dressing is expected to reduce the need for antibiotics, lower healthcare costs, and promote the use of chestnut by-products.