Related Experiment Videos
The ATG14-SIRT1 Axis Modulates NF-Κb Pathway Activation and Inflammatory Responses in Acute Lung Injury
Yali Wang1, Zilong Liu1, Haiying Ji1
1Department of Pulmonary and Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Immunological Investigations
|July 23, 2026
Summary
Autophagy regulator ATG14 acetylation impacts acute lung injury (ALI). Targeting the ATG14-SIRT1 pathway reduces inflammation, offering a potential therapeutic strategy for ALI.
Area of Science:
- Molecular Biology
- Immunology
- Cell Biology
Background:
- Acute lung injury (ALI) involves inflammation and tissue damage.
- Autophagy-related gene 14 (ATG14) is crucial for autophagy, but its role in ALI is unclear.
Purpose of the Study:
- To investigate the role of ATG14 in LPS-induced ALI.
- To elucidate the regulatory mechanism of ATG14 acetylation and its impact on inflammation.
Main Methods:
- LPS-induced ALI model in mice and lung epithelial cells.
- Assessed autophagy, ATG14 acetylation, NF-κB pathway, and cytokine production.
- Utilized ATG14 knockdown/overexpression and SIRT1 overexpression.
Main Results:
- LPS increased ATG14 expression, acetylation, and NF-κB activity, while decreasing SIRT1.
- SIRT1 interacted with ATG14, suppressing its acetylation.
- ATG14 inhibition or SIRT1 restoration reduced inflammation and cytokine production.
Conclusions:
- The ATG14-SIRT1 axis regulates autophagy-inflammation crosstalk in ALI.
- This axis presents a potential therapeutic target for ALI treatment.
Related Concept Videos
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...