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Published on: May 27, 2022
Validation of ERETIC-Based 1H-NMR for the Simultaneous Quantification of Cannabinoids in Complex Cannabis sativa
Rocío Figueroa1, Federico Arrighi2, Mauricio Cattaneo2,3
1Departamento de Química Orgánica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
Abstract:
Chromatographic methods for cannabinoid analysis currently face limitations, including lengthy procedures, artifact formation, and reliance on reference standards. This study establishes a robust quantitative 1H-NMR methodology for simultaneous analysis of acidic and neutral cannabinoids (THCA, CBDA, Δ9-THC, CBD, and CBN) in Cannabis sativa extracts. Two validated strategies, internal standard (butylated hydroxytoluene [BHT]) and ERETIC calibration, were compared, with diagnostic signals identified in interference-free spectral regions. The method demonstrated high precision (RSD < 5%), accuracy (recoveries 97%-102%), and low detection limits (MLOD 0.07 mg/mL). ERETIC outperformed traditional calibration by eliminating physical standards while maintaining equivalency. Successful chemotypic classification (THC:CBD ratios) confirmed practical applicability. The ERETIC-based NMR approach provides a rapid and reproducible alternative for comprehensive cannabinoid quantification without derivatization.
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