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Electrochemiluminescence Sensor for Uric Acid Detection Using Natural Enzyme-Confined Nanozyme Cascade
Lanlan Wu1, Xiaochun Deng1, Jiyang Liu1
1School of Chemistry and Chemical Engineering, Zhejiang Sci-Tech University, Hangzhou, China.
Summary
A novel electrochemiluminescence sensor detects uric acid (UA) using confined gold nanoparticles and bioenzymes. This sensitive method offers a new tool for diagnosing metabolic disorders in biological samples.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biomedical Sensing
Background:
- Metabolite detection, including uric acid (UA), is vital for diagnosing metabolic disorders.
- Existing biosensors often face challenges with sensitivity and stability.
Purpose of the Study:
- To develop a highly sensitive electrochemiluminescence (ECL) sensor for uric acid (UA) detection.
- To integrate bioenzyme and nanozyme reactions for enhanced sensing performance.
Main Methods:
- Synthesized amino-functionalized vertical mesoporous silica film (NH2-VMSF) on ITO via electrochemical-assisted self-assembly (EASA).
- Confined gold nanoparticles (AuNPs) within NH2-VMSF using electrochemical deposition.
- Utilized urate oxidase (UOx) for UA oxidation and luminol-H2O2 system for ECL detection.
Main Results:
- The NH2-VMSF/ITO electrode with confined AuNPs showed a 3.4-fold increase in ECL intensity.
- The sensor achieved a detection range of 0.1-20 μM with a low limit of detection (LOD) of 85 nM.
- Successfully detected UA in complex biological samples like urine.
Conclusions:
- The developed ECL sensor demonstrates high sensitivity and stability for UA detection.
- Nanoconfined AuNPs provide an effective microenvironment for nanozyme catalysis.
- This sensor shows promise for clinical diagnosis of metabolic disorders.
