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Published on: June 19, 2018
Integrated Single-cell Proteomic and Transcriptomic Landscape of Mouse Folliculogenesis
Hongchao Li1, Xinshuai Zhang1,2,3, Huimin Kang4
1Research Unit of Proteomics Driven Cancer Precision Medicine (Chinese Academy of Medical Sciences and Peking Union Medical College), State Key Laboratory of Medical Proteomics, National Center for Protein Sciences (Beijing), Beijing 102206, China.
Genomics, Proteomics & Bioinformatics
|July 23, 2026
Summary
This study reveals coordinated metabolic shifts during oocyte and granulosa cell development, crucial for female fertility. It uncovers key regulatory networks and signaling pathways impacting follicle growth and oocyte maturation.
Area of Science:
- Reproductive Biology
- Cellular Metabolism
- Molecular Endocrinology
Background:
- Folliculogenesis, essential for female fertility, involves complex oocyte-granulosa cell (GC) communication.
- Transcriptional regulation is well-studied, but the proteomic landscape of folliculogenesis remains largely unexplored.
Purpose of the Study:
- To comprehensively profile the proteomic and transcriptomic landscapes of oocytes and GCs during folliculogenesis.
- To elucidate cell type-specific molecular changes and regulatory networks governing follicle development.
Main Methods:
- Integrated dual-omics analysis of single oocytes and mini-bulk GCs across four follicular stages (secondary to preovulatory).
- Proteomic and transcriptomic profiling to characterize dynamic changes.
Main Results:
- Identified coordinated metabolic programs: oocytes accumulate lipids, while GCs boost energy and steroidogenesis.
- Revealed dynamic mitochondrial remodeling in oocytes and identified novel transcription factors and a SATB1-centered regulatory network.
- Demonstrated progressive increase in GDF9-BMPR2 signaling, indicating enhanced oocyte-GC communication.
Conclusions:
- Provides high-resolution insights into cell-specific proteomic and transcriptomic dynamics during folliculogenesis.
- Highlights the critical role of metabolic coordination and intercellular signaling in oocyte development and follicle growth.
- Suggests potential for novel fertility treatments and diagnostic strategies based on identified molecular mechanisms.
