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Updated: Aug 6, 2026
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
Published on: January 30, 2018
The Effect of Irradiation Setup Conditions on Cell Viability in Tumor Cell Cultures: An Investigation on Multi-well
Ayşegül Ünal Karabey1, Umut Diremsizoğlu1, Canan Öztürk2
1Department of Radiation Oncology, Kocaeli University Faculty of Medicine, Kocaeli, Türkiye.
Abstract:
Air gaps in multi-well cell culture plates can disrupt electronic equilibrium and introduce uncertainties in delivered dose during in vitro irradiation experiments. This study evaluated the impact of different bottom-up irradiation setups on short-term cell viability using an RW3 solid water phantom system. MCF-7 cells were irradiated with 6-MV photons (200-cGy) under four experimental configurations. One-way ANOVA demonstrated no statistically significant differences among setups [F(3,8)=0.503, p=0.691]. Tukey HSD post-hoc analysis confirmed the absence of significant pairwise differences (p>0.05). A subsequent power analysis (η2=0.159; Cohen's f=0.434) revealed an observed power of 0.15. These findings indicate that additional bolus or RW3 configurations do not significantly affect short-term cell viability when bottom-up geometry and appropriate buildup conditions are maintained.
