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Updated: Aug 6, 2026
Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies
Published on: October 6, 2022
Linalyl acetate alleviates inflammatory pain via OLFR482 activation in the mouse anterior cingulate cortex
Ban Feng1, Wei Guo2, Shu Zhao1
1Shanghai Key Laboratory of Anaesthesiology and Brain Functional Modulation, Translational Research Institute of Brain and Brain-Like Intelligence, Department of Anaesthesiology and Perioperative Medicine, Clinical Research Center for Anaesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai, China.
Introduction:
Chronic pain severely impairs quality of life and causes heavy socioeconomic burdens worldwide. Linalyl acetate (LA), a major volatile terpenoid ester from essential oils, possesses potential analgesic effects.
Methods:
A CFA-induced chronic inflammatory pain mouse model was established. Behavioral tests, immunofluorescence, whole-cell patch-clamp recording, RNA-seq, molecular docking, molecular dynamics simulation and AAV-mediated gene knockdown were applied to explore the analgesic mechanism of LA.
Results:
Systemic LA administration significantly alleviated inflammatory pain and suppressed neuronal hyperexcitability in the anterior cingulate cortex (ACC). Transcriptome analysis revealed marked upregulation of Olfr482 in the ACC of CFA-treated mice, and molecular docking confirmed the interaction between LA and OLFR482. Olfr482 knockdown largely abolished the analgesic effect of LA.
Discussion:
LA relieves chronic inflammatory pain via an olfactory-independent mechanism by activating OLFR482 and reducing neuronal hyperexcitability in the ACC. LA is a promising candidate for developing novel analgesic drugs.
