Molecular autopsy identifies the Na_V1.5 p.Leu96Pro variant causing sodium current loss-of-function in unexplained...

Molecular autopsy can identify candidate variants in unexplained sudden cardiac death (SUD), but functional evidence is often required to support pathogenic interpretation and family risk assessment. Here, we investigated the functional consequences of the SCN5A c.287 T > C (Na_V1.5_p.Leu96Pro) variant, identified in a 29-year-old man who died of SUD. Cascade screening identified additional relatives carrying the variant, with variable clinical expression. Sodium current (I_Na) was analyzed in heterologously transfected human embryonic kidney (HEK) tsA201 cells and in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) generated from two variant carriers and two non-carrier relatives. SCN5A transcript levels, Na_V1.5 membrane expression, and additional arrhythmia-associated genetic variation were also assessed. In HEK tsA201 cells, Na_V1.5_p.Leu96Pro produced no measurable I_Na when expressed alone, whereas co-expression with wild-type SCN5A caused an approximately 50% reduction in peak I_Na density. Cell-surface biotinylation showed preserved total and membrane Na_V1.5 expression, indicating that loss of current was not explained by impaired trafficking. hiPSC-CMs from both carriers showed reduced I_Na density compared with non-carrier relatives, despite no reduction in SCN5A transcript levels. Targeted sequencing did not identify variants explaining the differences between the two hiPSC-CM carriers, but revealed an additional SCN5A splice-site deletion in family members with more severe clinical manifestations. These findings show that Na_V1.5_p.Leu96Pro causes severe loss of sodium channel function and support the value of combining molecular autopsy, family evaluation, and functional studies for variant interpretation in SUD.