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Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
Published on: July 8, 2020
Lymphocyte-derived microRNA hsa-miR-150-5p as a marker of inflammation-related kidney function impairment in the
Sandra Van der Auwera1, Tim Lange2, Tilman Schmidt3
1Department of Psychiatry and Psychotherapy, University Medicine Greifswald, Greifswald, Germany; German Centre for Neurodegenerative Diseases (DZNE), Site Rostock/Greifswald, Greifswald, Germany.
Abstract:
Previous studies have demonstrated strong links between circulating microRNAs (miRNA), lymphocytes, and immune markers suggesting that miRNAs are key regulators of immune function and immune related diseases. We used the population-based SHIP-TREND cohort (N = 804) to investigate how specific plasma-based miRNAs relate to lymphocytes, cardiometabolic diseases, and clinical parameters. In linear regression, we identified miR-150-5p to be strongly lymphocyte-associated in both TREND waves. Beyond this, miR-150-5p showed significant associations with kidney disease and also with several clinical markers of renal function. Data indicate that these effects mark the transition from healthy to subclinical disease status and were independent of lymphocytes. We further validated the impact of miR-150-5p in independent publically available clinical datasets. Our findings suggest that the pathological effects of lymphocytes on kidney disease may be mediated through miR-150-5p, by regulating the expression of target genes and modulating inflammatory pathways. This highlights miR-150-5p as a potential early marker of subclinical kidney disease.
Insights
This study found miR-150-5p plasma levels are linked to kidney function and disease. It may serve as an early indicator for subclinical kidney disease, independent of lymphocytes.
Area of Science:
- Immunology
- Molecular Biology
- Nephrology
Background:
- MicroRNAs (miRNAs) are implicated in immune regulation and disease.
- Previous research suggests links between miRNAs, lymphocytes, and immune markers.
Purpose of the Study:
- To investigate the relationship between plasma miRNAs, lymphocytes, cardiometabolic diseases, and clinical parameters.
- To identify specific miRNAs associated with kidney function and disease.
Main Methods:
- Analysis of the population-based SHIP-TREND cohort (N=804).
- Linear regression to identify associations between plasma miRNAs and clinical parameters.
- Validation in independent public clinical datasets.
Main Results:
- miR-150-5p was strongly associated with lymphocytes in both study waves.
- miR-150-5p showed significant associations with kidney disease and renal function markers.
- These associations were independent of lymphocytes and indicated a transition to subclinical disease.
Conclusions:
- miR-150-5p may mediate the effects of lymphocytes on kidney disease by regulating gene expression and inflammatory pathways.
- miR-150-5p is a potential early biomarker for subclinical kidney disease.
- Further research into miR-150-5p's role in kidney pathology is warranted.
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