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Published on: April 11, 2016
A new method for the quantification of polysaccharide structural domain: Rapid quantification of type II
Shicheng Jiang1, Weihao Chen1, Yupeng Liu1
1The State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines, SATCM Key Laboratory for New Resources, Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai, 201203, PR China.
Abstract:
Type II arabinogalactans (AG-II) are biologically significant plant polysaccharides that exist both in free form and as side chains covalently bound within complex polysaccharides. However, the specific quantification of these bioactive AG-II domains in complex matrices remains a critical analytical challenge. Although the β-glucosyl Yariv reagent is a classic chemical reagent used in plant biology for the qualitative identification of arabinogalactan proteins (AGPs), its quantitative potential has been constrained by the lack of standardized protocols. Here, we established a spectrophotometric method based on the Yariv reagent for AG-II quantification. The method demonstrated high sensitivity and reproducibility, achieving a limit of detection (LOD) of 0.25 μg/mL, a limit of quantification (LOQ) of 0.75 μg/mL, and a recovery of 101.95%, indicating excellent applicability. To address the response discrepancy between the generic standard and the specific AG-II analyte, we implemented a "dual-standard strategy" and determined a conversion factor (1.188), successfully establishing a quantitative link between the accessible standard and the target AG-II polysaccharides. The method was applied to analyze Lycium barbarum samples from various origins, successfully revealing significant variations in the content of AG-II across different geographical regions, providing a novel technical pathway for the precise quantification of AG-II.
