Customizing 3D Hierarchically Macroporous Covalent Organic Framework Superstructures for Improved Biosensing...

Area of Science:

• Materials Science
• Electrochemistry
• Analytical Chemistry

Background:

• Optimized biosensing interfaces are crucial for sensitive electrochemical detection.
• Covalent organic frameworks (COFs) offer tunable properties for advanced materials.

Purpose of the Study:

• To construct macroporous COF nanostructures for enhanced biosensing interfaces.
• To develop a sensitive and stable electrochemical biosensor for organophosphorus pesticide detection.

Main Methods:

• Template-assisted assembly growth strategy for macroporous COF synthesis.
• Immobilization of acetylcholinesterase (AChE) onto the COF material.
• Electrochemical detection of organophosphorus pesticides (OPs).

Main Results:

• Achieved well-organized macroporous COF nanostructures suitable for electrode materials.
• Demonstrated efficient enzyme immobilization and improved mass/charge transfer.
• Developed a biosensor with sub-pg mL⁻¹ detection limits for OPs and high stability.
• Validated portable device performance in real-world samples (river water, leafy vegetables).

Conclusions:

• Macroporous COFs provide a superior platform for electrochemical biosensor development.
• The developed AChE-loaded COF biosensor shows practical applicability for environmental and food safety monitoring.
• This strategy advances high-performance portable electrochemical biosensors.