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MicroRNA-326 as a Tumor Suppressor Regulates the NOB1 Expression in Breast Cancer

Milad Noei1, Flora Forouzesh1, Fereshteh Abbasvandi2

  • 1Department of Genetics, TeMS.C, Islamic Azad University, Tehran, Iran, azad.ac.ir.

International Journal of Breast Cancer
|July 24, 2026
PubMed
Abstract

Insights

MicroRNA-326 (miR-326) expression is decreased in breast cancer, leading to increased NOB1 gene expression. This inverse relationship suggests miR-326 and NOB1 may serve as biomarkers for breast cancer diagnosis and therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Aberrant microRNA-326 (miR-326) expression is linked to diseases like cancer.
  • The NOB1 gene is crucial for 26S proteasome biogenesis and function.
  • miR-326 regulates NOB1 by targeting its mRNA, reducing NOB1 protein translation.

Purpose of the Study:

  • To assess miR-326 and NOB1 gene expression levels in breast cancer (BC) tissues.
  • To investigate the correlation between miR-326 and NOB1 expression in BC.
  • To compare expression profiles in BC, normal-adjacent, and healthy breast tissues.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) was employed to measure gene expression.
  • Expression levels of miR-326 and NOB1 were analyzed in 41 BC tissues, matched normal-adjacent tissues (NATs), and 8 healthy breast tissues.
  • Correlation analysis and Receiver-Operating Characteristic (ROC) curve analysis were performed.

Main Results:

  • miR-326 expression was significantly decreased in BC tissues compared to NATs and healthy tissues (p < 0.0001).
  • NOB1 expression was significantly increased in BC tissues compared to NATs and healthy tissues (p < 0.0001).
  • A significant negative correlation was found between miR-326 and NOB1 expression (p < 0.005).

Conclusions:

  • Decreased miR-326 expression in BC may lead to increased NOB1 gene expression.
  • The observed negative correlation suggests miR-326 regulates NOB1 expression.
  • Dysregulation of miR-326 and NOB1 may contribute to BC development, indicating their potential as diagnostic and therapeutic biomarkers.
Keywords:
NOB1breast cancermiR-326

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