In Situ Coenzyme-Based Transformable Polymers Powder With Instant-Tough-Adhesive Properties for Emergency Hemostasis,...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Polymer Chemistry

Background:

• Current tissue adhesives face challenges in wet adhesion, setting time, cytotoxicity, and lack of antimicrobial/pro-healing properties.
• Effective wound management requires solutions for hemorrhage control, infection prevention, and accelerated tissue repair.
• There is a need for advanced bioadhesives that perform reliably in challenging wound environments.

Purpose of the Study:

• To develop a self-stabilizing, water-responsive coacervate powder for rapid tissue sealing and improved wound healing.
• To create a versatile bioadhesive integrating antimicrobial and pro-healing functionalities.
• To address limitations of current tissue adhesives in managing traumatic wounds and promoting better healthcare outcomes.

Main Methods:

• Formulation of a coacervate powder (PLA/Na@TA/PLys) from coenzyme-based polymers, tannic acid, and ε-polylysine.
• Evaluation of ultra-rapid self-gelation kinetics upon hydration (<10 s).
• Assessment of adhesion strength (69.55 kPa) and burst pressure (216.23 mmHg) on wet tissues.
• Testing hemostatic efficacy in various bleeding models (<20 s) and evaluating antimicrobial/anti-biofilm activity.
• In vitro assessment of vascular endothelial cell functions and in vivo evaluation in full-thickness skin defects.

Main Results:

• The PLA/Na@TA/PLys powder demonstrated ultra-rapid gelation (<10 s) and strong adhesion to wet tissues.
• Achieved rapid hemostasis (<20 s) in severe bleeding models with no rebleeding, outperforming commercial hemostats.
• Exhibited broad-spectrum antibacterial and anti-biofilm activity.
• Promoted vascular endothelial cell viability, migration, and angiogenesis, accelerating wound healing in vivo.
• Showcased high biosafety, facile storage, and convenient application.

Conclusions:

• PLA/Na@TA/PLys is a versatile, water-responsive bioadhesive powder providing a one-stop solution for challenging wound management.
• The developed material offers rapid hemostasis, infection prevention, and enhanced tissue regeneration.
• This innovative bioadhesive holds significant translational potential for clinical applications in emergency care and tissue repair.