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Updated: Aug 6, 2026
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
Published on: October 11, 2021
Protocol for purifying iPSC-derived retinal pigment epithelial cells by sequential digestion and intrinsic
Fengming Yang1, Rui Li1, Xinrun Lv1
1School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China; The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
None:
Induced pluripotent stem cells (iPSC)-derived retinal pigment epithelium cells serve as a promising cell source for transplantation therapy in age-related macular degeneration. Here, we present a protocol for purifying the mature retinal pigment epithelium (RPE) cells post-differentiation. We describe steps for sequentially digesting cells with different enzymes and sorting them based on the autofluorescence of mature RPE cells, avoiding fixation and immunostaining. By maintaining high cell viability and purity, this cost-efficient protocol has broad implications for both in vitro research and transplantation applications.
