Related Experiment Video
Updated: Aug 6, 2026
On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System
Published on: July 18, 2025
Development and validation of a rapid and sensitive method for the detection of trifluoroacetic acid in honey
Zhixin Fei1, Ce Zhang2, Hang Zhang3
1Yunnan International Joint Laboratory of Public Health and Disease Control, Yunnan Center for Disease Control and Prevention (Yunnan Academy of Preventive Medicine), 1177 Xianghe street, Chenggong District, Kunming 650500, China; Yunnan Provincial Key Laboratory of Public Health and Biosafety & Health Laboratory Center, Yunnan Center for Disease Control and Prevention (Yunnan Academy of Preventive Medicine), 1177 Xianghe street, Chenggong District, Kunming 650500, China.
Abstract:
Residual trifluoroacetic acid (TFA) in food has emerged as a new concern, but studies on the detection of TFA remain limited. Herein, we developed and validated a liquid chromatography-tandem mass spectrometry method for the analysis of TFA in honey for the first time. A matrix-induced liquid-liquid extraction was designed for the extraction and isolation of TFA from honey. The suppressive effect of metal ions in the matrix, particularly Mg2+ and Ca2+, on the extraction of TFA was confirmed. Under optimal conditions, method validation demonstrated satisfactory selectivity, linearity, accuracy (98%-115%), and precision (2.0%-7.7%). In tests of the method on retail honey samples, TFA was detected in 36 out of 38 with a maximum concentration of 252 μg/kg. This study not only provides a rapid and sensitive method for the detection of TFA in honey, but it also elucidates the effect of metal ions and ionic strength on TFA extraction.
