Related Experiment Video
Updated: Aug 6, 2026

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
Published on: October 10, 2017
The Src family kinase YES regulates DNAJB6b chaperone activity to modulate tau aggregation in Alzheimer's disease
Meng-Chieh Lin1, Huan-Yun Chen2, Te-Sheng Lin3
1Department of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Abstract:
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by a gradual deterioration of memory. While its prevalence increases with age, the underlying mechanisms remain incompletely understood. Here, we examine the biological consequences of phosphorylation-mediated chaperone activity in AD-associated tau aggregates. Increased phosphorylation of DNAJB6b at Y53 is observed in the brain lysates of AD patients. Our research found that an activated Src family kinase, YES, phosphorylates Y53 in the J-domain of DNAJB6b and enhances the binding between DNAJB6b and Hsp70. The strengthened association between DNAJB6b and Hsp70 may lead to the accumulation of tau aggregates. These findings suggest that up-regulation of YES kinase modifies DNAJB6b and that the resulting alteration in DNAJB6b-dependent tau disaggregation may contribute to an increased risk of AD.
Related Concept Videos
Enzyme-linked Receptors
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Alzheimer Disease ll: Pathophysiology
Regulated Protein Degradation
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulation of Nuclear Protein Sorting
DNA Damage can Stall the Cell Cycle

