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Related Experiment Videos

Human amniotic fluid alpha-L-fucosidase.

J A Alhadeff, G L Andrews-Smith

    Clinical Genetics
    |November 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    This study investigated alpha-L-fucosidase in amniotic fluid, finding its properties consistent across different maternal ages and gestational periods. The enzyme is antigenically similar to human liver alpha-L-fucosidase.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Clinical Chemistry

    Background:

    • Alpha-L-fucosidase is an enzyme with various physiological roles.
    • Investigating amniotic fluid alpha-L-fucosidase provides insights into fetal development and potential diagnostic markers.
    • Understanding enzyme properties is crucial for biochemical and clinical applications.

    Purpose of the Study:

    • To investigate the activity and properties of alpha-L-fucosidase in human amniotic fluid.
    • To characterize kinetic, thermal, and antigenic properties of the enzyme.
    • To explore potential correlations with maternal age and gestational time.

    Main Methods:

    • Enzyme activity assay using 4-methylumbelliferyl substrate.
    • Kinetic characterization (Michaelis constant determination).

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  • Isoelectric focusing for enzyme heterogeneity.
  • Thermal stability assays.
  • Immunochemical analysis (double immunodiffusion, immunoprecipitation) using anti-liver alpha-L-fucosidase antibody.

    • Main Results:

    • Wide range of specific activity observed (0.20-1.45 nmol/min/mg protein), average 0.62 nmol/min/mg protein.
    • No clear correlation between enzyme activity and maternal age or gestational time, though trends were noted.
    • Enzyme properties (kinetic, immunochemical) were consistent regardless of maternal age or gestational time.
    • Isoelectric focusing revealed multiple enzyme forms (pH 5.0-6.8), with optimal activity near pH 5.0.
    • Apparent Km of 0.05 +/- 0.01 mM; enzyme thermostable at 37°C but labile at 45°C.
    • Amniotic fluid alpha-L-fucosidase is antigenically similar to human liver alpha-L-fucosidase.

    Conclusions:

    • Amniotic fluid alpha-L-fucosidase exhibits consistent kinetic and antigenic properties.
    • The enzyme is antigenically similar, if not identical, to human liver alpha-L-fucosidase.
    • While trends were observed, maternal age and gestational time did not significantly alter enzyme properties.