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HBV PreS/S gene mutations in patients with chronic hepatitis B

Bülent Çakal1,2, Bilger Çavuş3, Alp Atasoy3

  • 1Department of Medical Microbiology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. bulentcakal@yahoo.com.

Archives of Virology
|July 24, 2026
PubMed

Insights

Hepatitis B virus (HBV) PreS/S gene variations are linked to progressive liver disease. Specific mutations, particularly S207R/N/I/T and in the PreS2 region, are independently associated with advanced liver fibrosis in chronic hepatitis B patients.

Area of Science:

  • Hepatology
  • Virology
  • Molecular Biology

Background:

  • Progressive liver disease in chronic hepatitis B (CHB) is a significant health concern.
  • Hepatitis B virus (HBV) PreS/S gene variants are implicated in CHB pathogenesis.
  • Understanding the relationship between HBV genetic variations and liver histopathology is crucial for predicting disease progression.

Purpose of the Study:

  • To investigate the association between HBV PreS/S gene variations and liver histopathology.
  • To identify specific HBV PreS/S mutations linked to advanced liver fibrosis in CHB patients.

Main Methods:

  • Genomic DNA was extracted from 109 CHB patients.
  • The HBV PreS/S gene was amplified using PCR and sequenced via Sanger sequencing.
  • Amino acid substitutions, nonsense mutations, and deletions were analyzed against liver fibrosis stages.

Main Results:

  • A high mutation rate (14.9%) was observed across the HBV PreS/S gene, with the PreS2 region showing the highest frequency (27.27%).
  • Mutations L54P (PreS1), F130L/S (PreS2), S207R/N/I/T, and I208T (S gene) were significantly more common in patients with advanced fibrosis (F≥3).
  • The S207R/N/I/T mutation was identified as an independent risk factor for liver fibrosis, and PreS2 mutations correlated with higher fibrosis scores.

Conclusions:

  • The S207R/N/I/T mutation in the HBV S protein is independently associated with liver fibrosis progression in CHB.
  • PreS/S gene variations, particularly within the PreS2 region, play a role in the development and progression of liver fibrosis in chronic hepatitis B.

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