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Published on: October 27, 2023
Prevotella intermedia-Derived Extracellular Vesicles Promote Pulpitis
1Department of Endodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong Province, China.
Journal of Dental Research
|July 25, 2026
Summary
Bacterial extracellular vesicles from Prevotella intermedia induce pulpitis by causing macrophage necroptosis and IL-33 secretion, leading to ER stress in dental pulp cells. This reveals a new pathway in pulpitis pathogenesis.
Area of Science:
- Oral biology
- Microbiology
- Immunology
Background:
- Bacterial infection is the main cause of pulpitis, but mechanisms are unclear.
- Bacterial extracellular vesicles (BEVs) are key in infectious disease pathogenesis.
- The role of BEVs in pulpitis remains unexplored.
Purpose of the Study:
- To investigate the role of Prevotella intermedia-derived extracellular vesicles (Pi-EVs) in pulpitis.
- To elucidate the mechanisms by which Pi-EVs contribute to pulpitis development.
- To identify Pi-EVs as potential virulence factors in pulpitis.
Main Methods:
- Investigated uptake of Pi-EVs by macrophages.
- Analyzed Pi-EVs' effect on macrophage necroptosis and IL-33 secretion.
- Examined IL-33's impact on dental pulp cells (DPCs), including ER stress.
Main Results:
- Pi-EVs were internalized by macrophages, enhancing pathogenic effects.
- Pi-EVs induced macrophage necroptosis, increasing IL-33 secretion.
- IL-33 triggered ER stress in DPCs, promoting pulpitis progression.
Conclusions:
- Pi-EVs are identified as a novel virulence factor in pulpitis.
- A new pathogenic cascade involving Pi-EVs, macrophage necroptosis, IL-33, and ER stress in DPCs is revealed.
- This study offers insights into bacterial-host interactions and potential therapeutic targets for pulpitis.
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