Related Experiment Video
Updated: Aug 5, 2026

Glycan Node Analysis: A Bottom-up Approach to Glycomics
Published on: May 22, 2016
Transcriptional Scenario of Altered Glycosylation in HPV-Associated Cervical Cancer
Ashi R Thobias1, Kinjal A Patel1, Kruti A Mehta1
1Cancer Biology Department, The Gujarat Cancer & Research Institute, Asarwa, Ahmedabad, Gujarat 380 016 India.
Abstract:
Cervical cancer is one of the leading malignancies in women in India, with a high mortality rate and no available targeted therapy. This is due to a dearth of understanding of the underlying mechanisms regulating cervical carcinogenesis and the distinguished behavioral patterns of HPV-associated carcinomas. This study aims to understand the associations of HPV infection and cervical cancer with aberrant glycosylation, which is an enabling factor for other hallmarks of cancer. DNA and RNA were isolated from biopsy samples of 92 CC patients. HPV 16 and 18 infection was detected by endpoint type-specific PCR. The RNA samples were processed for expression analysis of the HPV 16-E6, HPV 16-E7 and HPV 16-E2 oncogenes. Analysis of the expression of glycosylated transcripts was performed via qPCR. HPV infection was correlated with the transcript levels of ST3GAL3, ST6GAL1, FUT5 and FUT8. In HPV 16- and 18-positive patients, the transcript levels of ST3GAL3, ST6GAL1 and FUT5 were increased, whereas FUT8 transcript levels were decreased. These data depict hyperglycosylation patterns associated with HPV infection. HPV-associated cancers show downregulation of core fucosylation and upregulation of ST3GAL3, ST6GAL1 and FUT5. The behavioral pattern of HPV-associated cancer could be due to its differential association with altered glycosylation.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s12291-024-01280-2.
Related Concept Videos
Epigenetic Regulation
X-chromosome...
Epigenetic Regulation
Abnormal Proliferation
Protein Glycosylation
Glycosylation occurs in...
Cancer-Critical Genes I: Proto-oncogenes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Loss of Tumor Suppressor Gene Functions
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
