miR-30b-5p Promotes Medulloblastoma Cell Proliferation by Regulating Stem Cell Factors NANOG, BMI1, Tumor Suppressor...

Medulloblastoma (MB), a malignant tumour arising in the brain, exhibits distinct microRNA expression patterns. One such microRNA, miR-30b-5p, has been relatively unexplored in its function within MB cells. This study investigates the regulatory role of miR-30b-5p in MB cells, particularly its influence on the Sonic Hedgehog signaling pathway through Gli1 mediation. Daoy MB cells were transfected with miR-30b-5p, a miR-negative control, or left Untreated. Cell proliferation assays, Reverse transcription PCR, and RT-qPCR were conducted to assess cell growth and target gene expression. The findings revealed that miR-30b-5p regulates DAOY cell growth at 48 and 72 h after transfection, but exhibits an inhibitory effect at 24 h compared to the miR-Negative Control group. Furthermore, the expression of genes GLI1, BMI1, P53, and NANOG was examined. At 24 h, these genes displayed lower expression in the miR-30b-5p group. Conversely, at 48 h, BMI1 and P53 expression continued to decrease, while GLI1 and NANOG showed increased expression compared to the miR-negative control group. In conclusion, the upregulation of miR-30b-5p resulted in decreased apoptosis and enhanced medulloblastoma proliferation. This effect was mediated through the positive regulation of Gli1 via the Sonic Hedgehog pathway, leading to increased expression of GLI1 and NANOG. These findings suggest a potential role for miR-30b-5p as a therapeutic target in MB treatment.