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Updated: Aug 6, 2026
07:26
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Phospholipase C-Delta1 Deletion Impairs Dermal Papilla Cell Function by Disrupting the HIF-1/MAPK Signaling Pathway
Jiawei Cai1, Bohao Zhao1, Yunpeng Li1
1College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.
Summary
Phospholipase C-delta 1 (PLCD1) is crucial for hair follicle development. Its absence impairs dermal papilla cell function by inhibiting the HIF-1/MAPK pathway, offering new therapeutic targets for hair loss.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Phospholipase C-delta 1 (PLCD1) is vital for hair follicle (HF) growth.
- Its regulatory role in dermal papilla cells (DPCs), key HF cells, is not well understood.
Purpose of the Study:
- To investigate the function of PLCD1 in DPCs.
- To elucidate the regulatory mechanism of PLCD1 in HF development.
Main Methods:
- CRISPR/Cas9 gene editing to create a PLCD1 knockout DPC model.
- Transcriptome and metabolomics analyses to compare PLCD1 knockout and wild-type DPCs.
- Western blotting to analyze Hypoxia inducible factor-1 (HIF-1) and Mitogen activated protein kinase (MAPK) signaling pathways.
Main Results:
- PLCD1 knockout significantly impaired DPC physiological functions.
- Transcriptome and metabolomics revealed a coordinated gene-metabolite regulatory network.
- PLCD1 deficiency inhibited the HIF-1/MAPK signaling pathway in DPCs.
Conclusions:
- PLCD1 deficiency impairs DPC function by inhibiting the HIF-1/MAPK pathway.
- The PLCD1-regulated gene-metabolite network is crucial for its mechanism.
- PLCD1 and its downstream network are potential therapeutic targets for hair-related diseases.
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