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Microfluidic synthesised PEGylated ascorbic acid-conjugated silver nanocarriers effectively downregulate oncogenes in
Ayesha Aftab1, Bashir Ahmad1, Shazia Bashir2,3
1Department of Biological Sciences, International Islamic University, Islamabad, Pakistan.
Aim:
We aim to formulate highly stabilised nanocarriers for the downregulation of oncogenes in IDC cells.
Methods:
A microfluidic system was operated for the synthesis of silver-tripolyphosphate nanoparticles (AgTPP-NPs) functionalised with polyethylene-glycol (PEG). They were characterised by UV-Vis spectroscopy, DLS, SEM, FTIR, and EDX. Biological evaluation included DPPH, MTT, clonogenesis, qRT-PCR, and western blot against MCF and MDA-MB-231.
Results:
AgTPP-NPs remained stable for 24 months, and particle size increased slightly from 78 ± 22nm to 88 ± 18nm over this period. PEGylation resulted in tuned (64 ± 15 nm) PEGylated ascorbic acid-conjugated-AgTPP (PAAT) nanocarriers, which have a significant (p < 0.0001) antioxidant property of 74.4% at 160 µg/mL (IC50=11.3 µg/mL). PAAT nanocarriers exhibited cytotoxicity of 81%. PAAT demonstrated a significant (p < 0.0001) downregulation of MYC and CCNE1 in IDC cells, validated by western blot analysis.
Conclusion:
Our study highlights the long-term stability of AgTPP-NPs, and PEGylation enhances the efficacy of AgTPP-NPs in terms of cytotoxicity and downregulation of oncogenes in IDC cells.
