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Updated: Aug 5, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
Published on: June 25, 2020
Transcriptomic changes in the jerboa supraoptic nucleus during osmotic stress: PCSK1 and PTPRN changes are conserved
Benjamin T Gillard1, Nabil Amor2, Michael P Greenwood3
1Molecular Neuroendocrinology Research Group, Bristol Medical School, Translational Health Sciences, Dorothy Hodgkin Building, University of Bristol, Bristol, UK.
Abstract:
The supraoptic nucleus (SON) is a hypothalamic region essential for the orchestration of osmoregulation in mammals. We sought to understand how gene expression in the SON is orchestrated in a rodent species adapted for survival in arid desert conditions. After an acclimatisation period, we compared the male and female trancriptomes of the Lesser Egyptian jerboa (Jaculus jaculus) SON under euhydrated, dehydrated, and rehydrated conditions. Gene expression in the jerboa SON was remarkably stable across the conditions with only 46 differentially expressed genes (DEGs) after dehydration and 4 DEGs after rehydration compared to controls; far fewer than previously shown to respond in the jerboa kidney and in the SON of mesic species. When comparing across species (jerboa, camel, and rat), the genes PCSK1 and PTPRN were consistently regulated under conditions of osmotic stress suggesting they have important conserved functions. The dehydration response in jerboa SON is tightly regulated. PCSK1 and PTPRN are identified as conserved components of the response, indicating their importance.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...