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Updated: Aug 5, 2026
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Fentanyl Analog Screening using LC-TIMS-TOF MS/MS
Published on: November 8, 2024
A Selective, Class-Specific Aptamer for Fentanyl Analog Screening
Linlin Wang1, Gabriel Castro1, Obtin Alkhamis1
1Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, North Carolina27607, United States.
Analytical Chemistry
|July 27, 2026
Summary
Researchers developed a novel aptamer test for rapid and accurate detection of fentanyl analogs. This new assay offers broad coverage and high specificity, potentially saving lives by controlling the spread of these dangerous substances.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Public Health
Background:
- Fentanyl and its analogs present significant public health and safety risks.
- Rapid and accurate detection methods are crucial for controlling the spread of these substances.
Purpose of the Study:
- To develop a highly specific and broadly reactive aptamer for detecting fentanyl analogs.
- To create a simple, rapid, and reliable detection assay for fentanyl analogs.
Main Methods:
- Systematic evolution of ligands by exponential enrichment (SELEX) was used to isolate a high-affinity aptamer.
- A single-step colorimetric dye-displacement assay was developed using the aptamer.
Main Results:
- The aptamer demonstrated high affinity and broad cross-reactivity to 139 fentanyl analogs.
- The assay showed excellent specificity against common interferents like diphenhydramine and methamphetamine.
- The aptamer-based assay's analog coverage rivals commercial immunoassay tests but with improved specificity.
Conclusions:
- Aptamers offer a promising platform for broad yet specific detection of structurally related molecules.
- The developed aptamer assay provides a rapid, simple, and cost-effective method for fentanyl analog detection.
- This technology has the potential to significantly improve public safety by enabling widespread, accurate fentanyl testing.
