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Updated: Aug 5, 2026
Dissection and Grading of Ovarian Development in Wild-Type Female Insects
Published on: July 14, 2023
Function of OBP11 and GOBP2 in Oviposition Preference of Plodia interpunctella Female Mediated by Nonanal
Chen Wang1, Dianxuan Wang1, Fangfang Zeng1
1School of Food and Strategic Reserves, Henan University of Technology/National Grain Industry (Storage Insect Pest Control) Technology Innovation Center/Grain Storage and Logistics National Engineering Research Center, Zhengzhou450001, China.
Abstract:
Odorant-binding proteins (OBPs) play essential roles in olfactory-mediated oviposition site selection in insects. The expression of OBPs in female Plodia interpunctella was analyzed after exposure to nonanal, and significantly upregulated OBPs were cloned, recombinantly expressed, and their binding affinities were determined. Female preference for nonanal was determined by RNA interference followed by Y-tube choice and two-choice oviposition assays. The expression levels of PintOBP11 and PintGOBP2 were significantly upregulated relative to the control. PintOBP11 was highly expressed in the head and exhibited strong binding affinity to nonanal. The expression levels of PintOBP11 and PintGOBP2 were decreased by 86.50% and 64.44% at 24 h, respectively, and female preference for nonanal was significantly decreased after simultaneous RNAi of PintOBP11 and PintGOBP2. These findings reveal the roles of PintGOBP2 and PintOBP11 in olfactory recognition underlying nonanal-mediated oviposition preference in female P. interpunctella, offering useful insights for attractant-based management of this pest.
