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Published on: August 23, 2024
Aziridination-Enabled Structural and Quantitative Lipidomics
Erin A Hirtzel1, Gopal Reddy Ramidi1, Xin Yan1
1Department of Chemistry, Texas A&M University, College Station77843, Texas, United States.
Journal of the American Society for Mass Spectrometry
|July 27, 2026
Summary
Olefin aziridination enhances lipid analysis using mass spectrometry (MS). This method precisely locates carbon-carbon double bonds and improves quantification for advanced lipidomics.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
Background:
- Chemical derivatization is crucial for lipid structural characterization via mass spectrometry (MS).
- Olefin aziridination has become a key technique in lipidomics for targeting carbon-carbon double bonds (C═C bonds).
Purpose of the Study:
- To review recent advances in aziridination-enabled MS methodologies for lipidomics.
- To highlight the impact of aziridination on structural elucidation and quantitative analysis of lipids.
Main Methods:
- Selective targeting of C═C bonds using various aziridination chemistries.
- Utilizing diagnostic fragmentation patterns for reliable C═C bond localization.
- Improving ionization efficiency for nonpolar lipid classes.
Main Results:
- Aziridination facilitates precise C═C bond localization and structural elucidation.
- The method enhances ionization efficiency, particularly for nonpolar lipids.
- Diverse aziridination reactions offer distinct advantages for lipid analysis.
Conclusions:
- Aziridination-assisted MS is a powerful strategy for precision lipidomics.
- This approach enables isomer-resolved capability and accurate lipid quantification.
- Recent developments offer significant improvements for analyzing complex biological samples.
