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Activity of Corallopyronin A Against ESKAPEE Pathogens: Potential and Translational Implications
Jennifer M Colquhoun1, Kara W Marshall1, Miriam Grosse2,3
1Research Service, Atlanta VA Medical Center, Decatur, GA 30033, USA.
Antibiotics (Basel, Switzerland)
|July 27, 2026
Summary
Corallopyronin A (CorA) shows selective activity against Acinetobacter baumannii, including rifampin-resistant strains. Its potential therapeutic utility is highlighted for biofilm infections and other applications despite serum binding challenges.
Area of Science:
- Antimicrobial resistance research
- Bacterial pathogenesis
- Drug discovery and development
Background:
- Corallopyronin A (CorA) is a bacterial RNA polymerase inhibitor with a distinct binding site from rifamycins.
- Its activity spectrum against ESKAPEE pathogens and therapeutic potential require further definition.
Purpose of the Study:
- To evaluate CorA's activity against ESKAPEE pathogens.
- To assess CorA's translational potential, including resistance, serum activity, in vivo efficacy, and biofilm disruption.
Main Methods:
- Broth microdilution assays with and without polymyxin B nonapeptide (PMBN).
- Benchmarking against rifampin (Rif), resistance and cross-resistance studies.
- In vivo efficacy in Galleria mellonella model and biofilm disruption assays.
Main Results:
- CorA inhibited Acinetobacter baumannii (MIC = 16-32 µg/mL), with enhanced susceptibility upon efflux disruption.
- Rif-resistant strains remained susceptible to CorA, indicating no cross-resistance.
- CorA showed biofilm disruption but limited in vivo efficacy in Galleria mellonella due to serum binding.
Conclusions:
- CorA exhibits selective activity against Acinetobacter baumannii and retains efficacy against rifampin-resistant strains.
- Serum binding impacts in vitro activity but does not preclude therapeutic utility in certain contexts.
- Potential applications include biofilm-associated infections and combination therapies.
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