Quantitative Assessment of Primary Colonizer Adhesion on Different Resin-Based Restorative Materials Using SYBR Green...

Area of Science:

• Dental Materials Science
• Microbiology
• Biofilm Research

Background:

• Oral biofilms are complex microbial communities crucial in dental health and disease.
• Understanding primary colonizer adhesion to restorative materials is key for preventing secondary infections and material degradation.
• Adhesive restorative materials form the interface between dental tissues and the oral environment, influencing microbial interactions.

Purpose of the Study:

• To investigate the adherence of primary colonizers to five different adhesive restorative materials.
• To compare the in vitro adhesion of specific oral bacteria to Admira Fusion, Clearfil AP-X, Durafill VS, Filtek Supreme XTE, and Venus Diamond.
• To identify potential material-specific differences in early oral biofilm development.

Main Methods:

• Five restorative materials were prepared into test specimens.
• Pellicle formation was induced using human saliva.
• Specimens were incubated with a mixed bacterial suspension of Streptococcus oralis, Streptococcus gordonii, Streptococcus sanguinis, Streptococcus mitis, and Actinomyces naeslundii.
• Bacterial adhesion was quantified using SYBR Green quantitative polymerase chain reaction (qPCR) to measure total genomic DNA.

Main Results:

• No significant differences in bacterial DNA were found for Streptococcus gordonii, Streptococcus oralis, and Actinomyces spp. across the materials.
• Filtek Supreme XTE exhibited significantly higher DNA concentrations for Streptococcus mitis compared to other materials.
• Significantly higher DNA concentrations of Streptococcus sanguinis were detected on Filtek Supreme XTE versus Clearfil AP-AX and Durafill VS.

Conclusions:

• The tested restorative materials demonstrate species-specific bacterial adhesion patterns.
• Filtek Supreme XTE showed increased adhesion for Streptococcus mitis and Streptococcus sanguinis.
• No significant differences in adhesion were observed for the other tested bacterial species, highlighting material-dependent biofilm formation.