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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Development of a Quantitative Detection System for Porphyromonas gingivalis Based on Immunochromatography
Kaoru Kobayashi1, Ryota Yamasaki1,2, Michihiko Usui3,4
1Division of Infections and Molecular Biology, Department of Advanced Pathophysiological Science, School of Dentistry, Kyushu Dental University, Kitakyushu 803-8580, Japan.
Dentistry Journal
|July 27, 2026
Summary
A new immunochromatography kit allows rapid, quantitative chairside detection of Porphyromonas gingivalis (Pg), a key bacterium in periodontitis. This reliable tool aids in diagnosing and managing periodontal disease and related systemic conditions.
Area of Science:
- Periodontology
- Microbiology
- Biotechnology
Background:
- Porphyromonas gingivalis (Pg) is central to periodontitis pathogenesis.
- Routine chairside detection of periodontopathic bacteria like Pg is lacking.
- Existing P. gingivalis tests lack practical chairside application.
Purpose of the Study:
- To develop a rapid, quantitative immunochromatography system for P. gingivalis detection.
- To establish a practical chairside diagnostic tool for periodontitis.
Main Methods:
- Development of an immunochromatography kit for P. gingivalis.
- Evaluation of the kit's performance using a dedicated measurement reader.
- Assessment of assay interference from oral substances and blood.
Main Results:
- The system achieved a detection limit of 2.6 × 10^4 CFU/mL for P. gingivalis.
- High correlations were observed between the dedicated reader and reference methods (R^2 = 0.9921).
- The assay demonstrated high precision (CV < 0.6%) and was minimally affected by common oral products.
Conclusions:
- The developed immunochromatography system offers a rapid and reliable chairside method for P. gingivalis detection.
- This tool has potential for diagnosing periodontitis and associated systemic conditions.
- The system addresses the need for practical chairside diagnostics in periodontology.
