Related Experiment Video
Updated: Aug 5, 2026

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
Published on: May 4, 2018
Nanoparticle-Mediated RNAi Delivery System Targeting the btCHS and btG6PI Genes in Bemisia tabaci
Jingjing Li1, Yinghan Zhao1, Chenchen Zhao1
1College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China.
Abstract:
RNA interference (RNAi) technology has emerged as a promising approach to developing biopesticides. However, its effectiveness in the field depends largely on developing efficient and stable systems that can deliver double-stranded (ds)RNA to the intended target. In this study, we describe a novel dsRNA delivery system based on a nanomaterial (C60/ APG), and evaluated its RNAi efficiency systematically by targeting the chitin synthase (btCHS) and glucose-6-phosphate isomerase (btG6PI) genes of the agricultural pest Bemisia tabaci. The results showed that compared with dsRNA alone (30% and 40%), the dsCHS + Nano and dsG6PI + Nano groups showed significantly lower survival rates (≈10% and 25%) at 10 days post-treatment, as nanocarrier-delivered dsRNA was absorbed by tobacco plants and triggered RNAi against the target genes, inhibiting B. tabaci development. These findings confirm the role of nanocarriers in improving RNAi efficacy and support the development of sustainable nano-RNAi strategies for crop pest control.
Related Concept Videos
Experimental RNAi
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
piRNA - Piwi-interacting RNAs
siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

