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Tissue-Specific Transcriptomics Uncover Exercise-Responsive Immune-Metabolic Regulatory Targets in Obesity
Yingfeng Chen1, Renqing Zhao2, Ji Ma3
1Department of Physical Education, Yangzhou Polytechnic University, Yangzhou 225009, China.
Metabolites
|July 27, 2026
Summary
Exercise reverses obesity-induced gene expression changes in adipose tissue by targeting immune-metabolic pathways. The study identified CCL2 as a key gene involved in exercise-mediated restoration of metabolic homeostasis.
Area of Science:
- Molecular Biology
- Immunology
- Metabolism
Background:
- Obesity disrupts immune-metabolic homeostasis.
- Molecular mechanisms of exercise-induced tissue restoration are unclear.
Purpose of the Study:
- Identify genes and pathways involved in exercise-mediated correction of obesity-related transcriptional disorders.
- Utilize multi-tissue transcriptome profiling and bioinformatic analysis.
Main Methods:
- Downloaded transcriptomic datasets (visceral adipose, subcutaneous adipose, skeletal muscle) from GEO database (mouse and human).
- Applied R programming for differential analysis, enrichment analysis (GO/KEGG), PPI networks, LASSO, and GSEA.
- Validated candidate genes using independent human adipose datasets.
Main Results:
- Exercise reversed obesity-induced transcriptional changes in adipose tissues, focusing on immune inflammation, lipid, and energy metabolism.
- Identified depot-specific pathway regulation and key hub genes for tissue remodeling.
- CCL2 demonstrated consistent expression trends across mouse and human adipose tissues.
Conclusions:
- Distinct tissue-specific responses to exercise observed: visceral adipose (inflammatory reversal), subcutaneous adipose (immune-metabolic reprogramming), skeletal muscle (energy metabolism adaptation).
- Immune-metabolic pathways are crucial for exercise-induced restoration in adipose tissues.
- CCL2 identified as a conserved candidate gene associated with exercise-responsive immune-metabolic pathways for anti-obesity research.
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