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Updated: Aug 5, 2026
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
A Novel Hemoglobin Variant, Hb Hefei [CD113(GTG > GGG)(Val > Gly); HBB:c.341T > G], Was Detected by Matrix-Assisted
Yueying Huang1, Rui Ji1, Weijie Xie1
1Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, People's Republic of China.
Abstract:
We report a novel hemoglobin (Hb) variant detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A 57-year-old female patient underwent a routine physical examination at our hospital. Her HbA1c results showed significant discrepancies among capillary electrophoresis (CE), high-performance liquid chromatography (HPLC), and boric acid affinity chromatography (BAC). CE yielded an HbA1c of 5.12%, whereas both HPLC and BAC produced significantly higher values (HPLC: 5.8-6.3%; BAC: 5.96%). Neither the chromatograms nor the electropherogram showed any abnormalities. MALDI-TOF MS identified a variant β-globin chain with a mass-to-charge ratio (m/z) of 15,826 Da. Sanger sequencing revealed a novel heterozygous missense mutation in the HBB gene [CD113(GTG > GGG)(Val > Gly); c.341T > G]. We named it Hb Hefei after the proband's birthplace.
