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OsPUP1 Modulates Cytokinin Distribution and Antioxidant Defense to Regulate Heat Stress Tolerance in Rice
Shujie Wang1,2, Qiang Yu1,2, Junwen Zhang1
1Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance, College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
Antioxidants (Basel, Switzerland)
|July 28, 2026
Summary
Overexpressing the OsPUP1 gene in rice severely inhibits growth and increases oxidative damage under heat stress, indicating OsPUP1
Area of Science:
- Plant Biology
- Molecular Biology
- Stress Physiology
Background:
- Heat stress is a major constraint on rice (Oryza sativa) growth.
- The OsPUP1 gene, a purine transporter, is known to be involved in cytokinin (CK) transport.
- The specific role of OsPUP1 in the plant's response to heat stress remains unclear.
Purpose of the Study:
- To elucidate the role of the OsPUP1 gene in rice's response to heat stress.
- To investigate how OsPUP1 influences plant adaptation to high temperatures.
Main Methods:
- Analysis of OsPUP1 overexpression lines under heat stress conditions.
- Measurement of oxidative damage markers, including peroxides and malondialdehyde (MDA).
- Assay of antioxidant enzyme activities, such as superoxide dismutase (SOD) and peroxidase (POD).
Main Results:
- Overexpression of OsPUP1 resulted in significant growth inhibition and increased oxidative damage under heat stress.
- OsPUP1 overexpression lines showed higher accumulation of peroxides and MDA.
- Activities of antioxidant enzymes SOD and POD were significantly reduced in OsPUP1 overexpression lines.
Conclusions:
- OsPUP1 plays a crucial role in regulating rice thermotolerance.
- OsPUP1 modulates cytokinin transport, affecting antioxidant defenses and heat stress response.
- Cytokinin distribution is vital for plant adaptation to heat stress.
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