Multiplex PCR Fluorescence Method for Detection of Genetically Modified Maize Strains

Area of Science:

• Agricultural Biotechnology
• Molecular Diagnostics
• Food Safety Analysis

Background:

• Genetically modified (GM) crops present regulatory and traceability challenges.
• Traditional PCR methods lack the high-throughput, specificity, and sensitivity needed for GM detection.

Purpose of the Study:

• To develop a multiplex fluorescence PCR-capillary electrophoresis platform for simultaneous GM detection.
• To enhance the efficiency and accuracy of transgenic organism identification in food and feed.

Main Methods:

• Developed a platform labeling endogenous and exogenous gene primers with distinct fluorescent groups.
• Utilized multiplex PCR and capillary electrophoresis for simultaneous detection of 27 GM-related genes and events.
• Validated the platform with GM maize events and mixed samples.

Main Results:

• Accurate identification of all seven tested GM maize events.
• Precise discrimination of a mixed sample containing five GM maize varieties.
• Achieved a detection sensitivity of 0.1% for GM content.

Conclusions:

• The developed platform simplifies workflows while maintaining high analytical performance for GM detection.
• Combines multicolor fluorescence and capillary electrophoresis for robust, high-resolution multiplex detection.
• Offers a novel tool for rapid transgenic crop detection in food safety, import/export inspection, and biosafety surveillance.