Research Progress on the Regulatory Mechanisms of Salt-Stress Response and Functional Genes in Populus

Soil salinization represents one of the most severe abiotic constraints on global forest productivity. Populus, the most widely cultivated fast-growing timber tree and a premier model woody plant, exhibits striking intrageneric variation in salt tolerance-from the extremely halophytic Populus euphratica to highly salt-sensitive cultivated clones. Understanding the molecular basis of this variation has profound implications for saline-alkali land reclamation and salt-tolerant variety breeding. This review systematically synthesizes current knowledge on Populus salt-stress responses, covering three primary injury mechanisms (osmotic stress, ionic toxicity, and oxidative damage) and the corresponding physiological countermeasures. We further survey functional genes across four major categories: ion transporters, osmotic-adjustment enzymes, antioxidant-defense components, and transcription factors. Crucially, we extend beyond the herbaceous-plant paradigm by examining salt-tolerance strategies that are specific to the woody architecture of Populus: long-distance radial and axial Na⁺ transport through tall stems, salt sequestration in senescent bark and wood parenchyma, and deep-root ion exclusion strategies. Comparative insights from other woody genera are incorporated to highlight convergent and divergent mechanisms. On this basis, we propose an integrated multi-level regulatory model in which Na⁺ compartmentalization/efflux serves as the core, ROS homeostasis as the key regulatory axis, and osmotic adjustment as the auxiliary strategy. Outstanding challenges-including unresolved primary salt-signal perception, insufficient pathway integration, and limited in planta gene-function verification-are critically assessed, and future research priorities encompassing multi-omics integration, CRISPR-based gene editing, and natural-population genomics are outlined.