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Updated: Aug 5, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
Published on: September 17, 2016
LcCOL7 and LcCOL8 Negatively Regulate Plant Flowering Independent of Day Length
Tingting Yan1, Yukun He1, Tianyi Tang1,2
1Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences/Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Tropical Fruit Tree Biology of Hainan Province, Haikou 571100, China.
Abstract:
CONSTANS-LIKE (COL) genes are pivotal regulatory components in the photoperiodic flowering pathway of plants. These genes can be regulated by both photoreceptors and the circadian clock, modulating plant flowering responses under specific day lengths by regulating florigen levels. However, the COL gene family in Litchi chinensis Sonn. has not yet been characterized. In this study, we identified eight COL family members in litchi and classified them into three subgroups based on phylogenetic analysis. The analysis of cis-regulatory elements within the promoters of LcCOLs revealed a wide distribution of elements associated with light, hormone, and stress responses. Transcript expression profiling indicated that most LcCOLs exhibited relatively high expression levels in leaf buds, leaves, and young fruits. Diurnal expression analysis under natural photoperiod conditions revealed that the expression peaks of all LcCOLs, with the exception of LcCOL1, occurred during the nighttime. The heterologous overexpression of LcCOL7 and LcCOL8, the closest homologs to AtCO, in Arabidopsis thaliana significantly delayed the flowering time under both long-day (LD) and short-day (SD) conditions, indicating that these genes act as repressors of flowering. This study provides a foundational basis for elucidating the molecular mechanisms underlying litchi flowering regulation and identifies promising candidate genes for the molecular breeding of litchi flowering-related agronomic traits.
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