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Abscisic Acid Stimulates Ethylene Biosynthesis by Repressing the Expression of FaMADS1 in Postharvest Strawberry
Renchi Chen1,2,3, Yuhua Yan1,2,3
1Institute of Food Science, Wenzhou Academy of Agricultural Science, Wenzhou 325006, China.
Abstract:
Abscisic acid (ABA) and ethylene are both essential regulators of strawberry fruit (Fragaria × ananassa Duch.) ripening. This study investigated the interplay between ABA and ethylene, revealing that ABA downregulates FaMADS1, thereby de-repressing ethylene biosynthesis genes. Functional validation via FaMADS1 silencing and over-expression confirmed that FaMADS1 acts as a negative regulator of ethylene biosynthesis key genes (FaSAMS1, FaACS1, and FaACO1). Furthermore, dual-luciferase assays demonstrated FaMADS1's direct suppression of the promoters of key ethylene biosynthesis genes. Collectively, these results elucidated a novel mechanism wherein ABA and ethylene coordinate ripening via FaMADS1-mediated transcriptional repression. This ABA-FaMADS1-ethylene regulatory cascade provides a theoretical basis for targeted postharvest regulation of strawberry ripening, laying a foundation for developing low-cost, hormone-based preservation technologies and molecular breeding strategies to alleviate rapid quality deterioration and economic losses of harvested strawberry.
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